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Measuring crossovers with fluorescent pollen

The Henderson laboratory have published a paper in Nature Protocols describing measurement of crossover recombination using flow cytometry of fluorescent pollen, in collaboration with the laboratory of Greg Copenhaver (University of North Carolina, Chapel Hill). The major advantage to this protocol is that 50,000 pollen grains can be counted in 10 minutes, which would otherwise take 25 hours of manual scoring. This approach will accelerate study of crossover recombination in plants, which is an important tool for crop breeding.

Read online: doi:10.1038/nprot.2013.131