University of Cambridge
Cambridge CB2 3EA
My PhD can be divided into three main goals, which are contained under the umbrella title ‘Phased small RNAs during tomato development’:
Part I: NLRs, a novel TAS gene, and the regulation of defence response in Solanaceae
This research is focused on an ancient microRNA super family and the silencing cascade of a set of disease resistance proteins, NLRs, that these miRNAs trigger. We have evidence suggesting the presence of a novel TAS gene specific to Solenaceae. The production of phasiRNAs from this ncRNA transcript would allow pathogen-inducible regulation of NLRs independently of a basal expression of resistance genes. The main aim of my research is to dissect the range of these silencing pathways and their possible interactions.
Part II: Characterization of a pre-meiotic specific Argonaute involved in gamete development, and its interaction with a newly described class of phased small RNAs
Part III: Effects of transposable element activation and derived siRNAs in the shoot apical meristem
Canto-Pastor A, Molla-Morales A, Ernst E, Dahl W, Zhai J, Yan Y, Meyers BC, Shanklin J, Martienssen R. (2014) Efficient transformation and artificial miRNA gene silencing in Lemna minor. Plant Biol. doi:10.1111/plb.12215
Martienssen, R., Molla-Morales, A., Canto-Pastor, A., Ernst, E., Shanklin, J., & Yan, Y. (2014). Transformation of duckweed and uses thereof. U.S. Patent Application No. 14/913,057.