Recent evidence points to chloroplast involvement in Ca2+ signalling events in the cytosol. The chloroplast is now implicated in control of guard cell movements and gas exchange through sensing changes in stromal Ca2+ (1,2). Stromal Ca2+ levels also change during light/dark transition and could be involved in signalling for control of photosynthesis (3). How is stromal Ca2+ regulated? This project will involve characterising the Ca2+ channels of the Arabidopsis thylakoid membrane and examining their regulation by reactive oxygen species. Channel mutants will be transformed to express apoaequorin in the stroma so that their role in regulating stromal Ca2+ can be deduced. Mutants will also be phenotyped to assess impact on photosynthesis, morphology, gas exchange and productivity.
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