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L7 - Imaging: green fluorescent protein Lecture handout - review: Baird, G.S, David A. Zacharias, and Roger Y. Tsien (2000) Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral. PNAS USA 97:11984–11989. Cutler, S. R., D. W. Ehrhardt, et al. (2000). “Random GFP::cDNA fusions enable visualization of subcellular structures in cells of Arabidopsis at a high frequency.” Proc Natl Acad Sci U S A 97(7): 3718-23. Gross, L.A., Geoffrey S. Baird, Ross C. Hoffman, Kim K. Baldridge, and Roger Y. Tsien (2000) The structure of the chromophore within DsRed, a red fluorescent protein from coral. PNAS 97: 11990–11995 Haseloff, J., Emma-Louise Dormand and Andrea H. Brand (1999) Methods in Molecular Biology: Protocols in Confocal microscopy Chapter 17. Live imaging of green fluorescent protein Heikal, A.A., Samuel T. Hess, Geoffrey S. Baird, Roger Y. Tsien, and Watt W. Webb (2000) Molecular spectroscopy and dynamics of intrinsically fluorescent proteins: Coral red (dsRed) and yellow (Citrine) PNAS 97: 11996–12001. Weber, W., Helms, V., McCammon, J.A., and Langhoff, P.W. (1999) Shedding light on the dark and weakly fluorescent states of green fluorescent proteins. Proc. Natl. Acad. Sci. USA 96: 6177–6182. Weiss, S. (2000) Shattering the diffraction limit of light: A revolution in fluorescence microscopy? PNAS 97: 8747–8749 Wiedenmann, J., Carsten Elke, Klaus-Dieter Spindler, and Werner Funke (2000) Cracks in the ß-can: Fluorescent proteins from Anemonia sulcata (Anthozoa, Actinaria). PNAS USA 97: 14091–14096. Heim, R., Prasher, D. C. and Tsien, R. Y. (1994) Wavelength mutations an posttranslational autoxidation of green fluorescent protein. Proc. Natl. Acad. Sci USA 91, 12501-12504. Ogawa, H., Inouye, S., Tsuji, F. I., Yasuda, K. and Umesono, K. (1995) Localization , trafficking and temperature-dependence of the Aequorea green fluorescent protein in cultured vertebrate cells. Proc. Natl. Acad. Sci. USA 92, 11899-11903 Yeh, E., Gustafson, K. and Boullianne, G. (1995) Green fluorescent protein as a vital marker and reporter of gene expression in Drosophila. Proc. Natl Acad Sci. USA. 92, 7036-7040. Ma, X., Ehrhardt, D. W. and Margolin, W. (1996) Colocalization of cell division proteins FtsZ and FtsA to cytoskeletal structures in living Escherichia coli cells by using green fluorescent protein. Proc. Natl. Acad. Sci. USA 93, pp. 12998—13003 Moores, S. L., Sabry, J. H. and Spudich, J. A. (1996) Myosin dynamics in live Dictyostelium cells. Proc. Natl. Acad. Sci. USA 93, 443—446. Reichel, C., Mathur, J. Eckes, P. Langenkemper, Koncz, C., Schell, J., Reiss, B and Maas, C. (1996). Enhanced green fluorescence by the expression of an Aequorea victoria green fluorescent protein mutant in mono- and dicotyledonous plant cells. Proc. Natl. 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(1998) PRIM: Proximity imaging of green fluorescent protein-tagged polypeptides. Proc. Natl. Acad. Sci. USA 95, 12312—12316. Godwin, A. R., Stadler, H. S, Nakamura, K. and Capecchi, M. R. (1998) Detection of targeted GFP-Hox gene fusions during mouse embryogenesis Proc. Natl. Acad. Sci. USA 95, pp. 13042—13047. Jessen, J. R., Meng, A. McFarlane, R. J., Paw, B. H. Zon, L. I., Smith, G. R. and Lin, S. (1998). Modification of bacterial artificial chromosomes through Chi-stimulated homologous recombination and its application in zebrafish transgenesis. Proc. Natl. Acad. Sci. USA 95, 5121—5126. Llopis, J., McCaffery, J. M., Miyawaki, A. Farquar, M. G. and Tsien, R. Y. (1998). Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins. Proc. Natl. Acad. Sci. USA 95, 6803—6808. Nishiuchi, Y., Inui, T., Nishio, H., Bodi, J., Kimura, T., Tsuji, F. I. And Sakakibara. (1998) Chemical synthesis of the precursor molecule of the Aequorea green fluorescent protein, subsequent folding, and development of fluorescence. Proc. Natl. Acad. Sci. USA 95, 13549—13554. Reichel, C. and Beachy, R. N. (1998) Tobacco mosaic virus infection induces severe morphological changes of the endoplasmic reticulum. Proc. Natl. Acad. Sci. USA 95, 11169—11174. Szczesna-Skorupa, E., Chen, C. D., Rogers, S. and Kemper, B. (1998). Mobility of cytochrome P450 in the endoplasmic reticulum membrane. Proc. Natl. Acad. Sci. USA 95, 14793—14798. Baird, G. S., Zacharias, D. A. and Tsien, R. Y. (1999) Circular permutation and receptor insertion within green fluorescent proteins. Proc. Natl. Acad. Sci. USA 96, 11241-11246. Bryant, Z., Subrahmanyan, L., Tworoger, M., LaTray, L. Liu, C.-R. Li, M.-J., van den Engh, G. and Ruohola-Baker, H. (1999). Characterization of differentially expressed genes in purified Drosophila follicle cells: Toward a general strategy for cell type-specific developmental analysis. Proc. Natl. Acad. Sci. USA 96, 5559—5564. MacMorris, M., Zorio, D. A. R. and Blumenthal, T. (1999) An exon that prevents transport of a mature mRNA. Proc. Natl. Acad. Sci. USA 96, 3813—3818. Raskin, D. M. and De Boer, P. A. J. (1999) Rapid pole-to-pole oscillation of a protein required for directing division to the middle of Escherichia coli. Proc. Natl. Acad. Sci. USA 96, 4971—4976. Sun, B., Xu, P. and Salvaterra, P. M. (1999) Dynamic visualization of nervous system in live Drosophila. Proc. Natl. Acad. Sci. USA. 96, 10438—10443 Weber, W., Helms, V., McCammon, J. A., Langhoff, P. W. (1999). Shedding light on the dark and weakly fluorescent states of green fluorescent proteins. Proc. Natl. Acad. Sci. USA 96, 6177—6182. Yang, M., Baranov, E., Jiang, P., Sun, F. X., Li, X.M., Li, L., Hasegawa, S., Bouvet, M., Al-Tuwaijri, M., Chishima, T., Shimada, H., Moossa, A.R., Penman, S., and Hoffman, R.M. (2000) Whole-body optical imaging of green fluorescent protein-expressing tumors and metastases. Proc. Natl. Acad. Sci. USA 97, 1206-1211. |